"At this stage of my career I feel it is time to step aside and give younger players the privileged opportunity to play for Wales," said Pembridge.
"It has been both an honour and a privilege to represent my country at the highest level.
"I would like to thank all at the Football Association of Wales, the managers I have worked under, the players, and the fans for their support.
"The past 13 years have been very special and have given me some of the most memorable moments of my career.
"I wish everyone involved all the very best for the future."